Human CD8 is a heterodimeric protein consisting of an alpha and beta chain, each about 33 kd. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the alpha 2 and alpha 3 domain of MHC Class I molecules (3, 4).
Antibodies 14 and UCHT4 (cat #153-020) each bind to a distinct epitope of CD8 and are suitable as a matched pair for EIA using UCHT4 as capture and Biotinylated clone 14(cat# 154-030) as detector.
Isotype: Murine IgG1 kappa
Immunogen: Human thymocytes
Specificity: Antibody 14 recognizes the CD8 alpha chain of approximately 33 kd (1).
1. Leukocyte Typing II (E.L. Reinherz, et al, eds.) Springer Verlag, New York, (1986)
2. R. Zamoyska, (1994) Immunity 1: 243-246.
3. Leukocyte Typing V ( S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 353-356.
4. J. Sun, et al, (1995) J Exp Med 182: 1275-1280.