The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down-regulated by mitogenic stimulation.2  BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells.3,4  Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells 1.  Cell surface BAFF can be cleaved to form a soluble trimeric molecule.

Molecular Structure: A soluble molecule consisting of 151 (VQGPEET…c-term) aminoacids of the extracellular domain of human BAFF fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric weight of 38.5 kd. A portion of the extracellular region of BAFF  was truncated to amino acid 135 to eliminate a potential protease cleavage site.

 Transfectant Cell Line: CHO BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR (8, 9) .

Functional Application: Human BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA (8, 9).

References:

1) Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189(11):1747-1756.

2) Shu, H.B., H. Johnson, W.H. Hui. J Leukoc Biol 1999, 65:680-683.

3) Marsters, S.A., A. Ashkenazi, et al. 2000, Curr Biol 10:785-788.

4) Xia, X., H. Hsu, et al. 2000, J Exp Med, 192(1): 137-143.

5) Thompson J.S., C. Ambrose, et al. Science 2001, 293: 2108-2111.

6) Roschke, V, T.S. Migone, et al. J Immunol. 2002, 169: 4314-4321.

7) MacLennan, C.M., C.G. Vinuesa, 2002, Immunity 17:235-238.

8) Landau DA, P Cacoub, et al. (2009) Ann Rheum Dis 68(3): 337-44. PMID: 18434450.

9) Moreaux J, K Tarte, et al. (2004) Blood 103:3148-3157. PMID: 15070697.

10.  EY Moon, S Lee, et al. (2011) Intl Immunopharmacol 11(10): 1523-1533. https://doi.org/10.1016/j.intimp.2011.05.009

USD $375.00

In cart: 0
= $375.00
Catalog #: 525-030
Form: Biotin
Size: 25 µg
Alternate Name: TALL-1, Blys, THANK, B-cell Activating Factor, TNFSFL13
Applications: FC, EIA

Downloads

Other Forms