Human CD252 (CD134L, OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD252 binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection (5,6,7,8). CD252-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.
Molecular Structure: A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 132 (c-term) amino acids of the extracellular domain of human CD252, with a predicted (non glycosylated) monomeric weight of 34.1 kd. Extracellular mature murine CD8alpha: (28)KPQAPEL….GLDFACD(194); Linking amino acids: TR; extracellular human CD252: (52)VSHRYPR….NPGEFCVL(183).
Transfectant Cell Line: CHO
Functional Application: CD134L-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.
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