Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells.  Interaction of CD154 and CD40 is essential for isotype switching in B cells.  Known genetic defects that alter this interaction lead to impaired immune system function (1).  CD154  has been shown to be hyperexpressed by B and T cells in SLE patients (4).  CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells and macrophages indicating a possible role for the CD40-CD154 immunoregulatory signaling mechanism during inflammation and immunity in atherogenesis (6).

Isotype: Murine IgG1

Immunogen: Human sgp39 fusion protein

Specificity: Antibody 24-31 immunoprecipitates a CD154 (gp39) molecule of about 39 kd.  This clone has been reported to cross react with Cynomolgus and Rhesus Monkey CD154.

Functional Application: The antibody 24-31 will block MLR, sgp39 induced human B cell proliferation and T cell dependent B cell differentiation (9).

References:

1.  D. Gray, et al, (1994) Seminars in Immunol  6: 303-310.

2.  F. Pietravalle, et al, (1996) J Biol Chemistry  271: 5965-5967.

3.  R.J. Noelle, (1996) Immunity  4: 415-419.

4.  A. Desai-Mehta, et al, (1996) J Clin Invest  97: 2063-2073.

5.  I.S. Grewal and R.A. Flavell, (1996) Immunol Today  17: 410-414.

6.  F. Mach, et al, (1997) Proc Natl Acad Sci USA  94:1931-1936.

7. F. Pietraville, et al, (1996) Eur J. Immunol 26:725-728.

8. U.G. Wagner, C. Weyland, et al, (1998) J Immunol 161: 6390-6397.

9. M. Epeldegui, O. Martinez-Maza, et al. (2010) PLOS one 5(7): e11448. PMID: 20625427.

10. B Barnhart, L R Covey, et al. (2000) Immunology 99(1): 54-61. PMID: 10651941

USD $275.00

In cart: 0
= $275.00
Catalog #: 353-030
Form: Biotin
Size: 100 µg
Alternate Name: CD40L, CD40 Ligand, gp39
Clone: 24-31
Applications: FC, EIA, WB(2,6,7), IHC(8)

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