Human CD32 exists in at least six isoforms originating from three different genes (Fc gamma RII A, B, and C) .  The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses.

Isotype: Murine IgG1k

Immunogen: Human K562 tumor cells and L cells transfected with human Fc gamma RII

Specificity: Antibody 7.3 reacts with a domain 2 epitope of all CD32 isoforms (1). It exhibits preferential binding to  Fc gRIIb (inhibitory) isoform transfectants(2, 5).

Functional Application: Intact and F(ab‘)2  7.3 antibody blocks immune complex binding (4).  Incubation with anti-CD32 F(ab‘)2 inhibits Fibrocyte differentiation(6), and inhibits pro inflammatory cytokine secretion by SLE patient derived monocytes (7).

References:

1.   Leukocyte Typing V (S.F. Schlossman, et al, eds.)  Oxford University Press, Oxford  (1995)  p. 823-836.

2.  Liu Y, L Pricop, et al.  (2005) J Leukocyte Biol 77: 767-776 PMID:15703199

3.  S. von Gunten, H.U. Simon, et al. (2006) Blood 108(13): 4255-425

4. Laborde EA, M Vulcano, et al. (2007) J Immunol 179(1): 673-681.

5. Ierino FL, P M Hogarth, et al. (1993) J Immunol 150(5): 1794-1803.

6. Pilling D, NM Tucker, RH Gomer (2006) J Leuk Biol 79(6): 1242-1251.

7. Thanei S,  M Trendelenburg  (2016) J Immunology 196: 2063–2074. PMID: 26829984

USD $695.00

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Catalog #: 181-580
Form: Fab
Size: 100 µg
Alternate Name: Fcγ RII, Fc gamma RII
Clone: 7.3
Applications: FC

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