Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphatidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages.
Isotype: Murine IgG1
Immunogen: Human polymorphonuclear leukocytes
Specificity: Antibody 3G8 recognizes both allelic forms of human CD16 (1, 4). It is cross reactive with cynomolgus CD16 (7).
Functional Application: Antibody 3G8 blocks binding of complexed IgG to CD16 (2, 5). It is not suitable for Western blot. Ancell 3G8 F(ab‘2) has been used to upregulate Mac-1 expression in neutrophils (8).
1. H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA 79: 3275-3279.
2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597.
3. G. Trinchieri & N. Valiante, (1993) Natural Immunity 12: 218-234.
4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 805-814.
5. J.C. Edberg & R.P. Kimberly, (1997) J Immunol 159: 3849-3857.