Ancell Corporation

anti-CD105 fluorescent conjugates for phenotypic analysis of Mesenchymal Stem Cells

Ancell Corporation — Fri, 14 Jun 2013 00:00:00 -0500

Featured product: Ancell anti-CD105/FITC clone SN6

In these studies, Mesenchymal Stem/Progenitor cells show potential for repairing damaged joint tissue, and restoring liver function. After enriched by sorting, and expansion in culture, the phenotype of resulting stem cell populations are: CD105+, CD29+, CD166+, CD44+, CD45-, CD31-.

1) “Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes^” A Banas, T Ochiya, et al. (2007) Hepatology 46: 219-228.

2)“Identification and Clonal Characterisation of a Progenitor Cell Sub-Population in Normal Human Articular Cartilage” R Williams, HE McCathy, et al. (2010) PLoS ONE 5(10): e13246 doi:10.1371.

3)“Identification of Mesenchymal Progenitor Cells in Normal and Osteoarthritic Human Articular Cartilage” S Alsalameh, M Lotz, et al. (2004) Arthritis & Rhematism 50(5): 1522-1532.

Related Ancell Products

Biotinylated mAb cocktail effective for isolating Low Density Granulocytes from SLE patient blood

Ancell Corporation — Thu, 02 May 2013 00:00:00 -0500

Featured product: Ancell Biotinylated mAb cocktail effective for Negative selection of Low Density Granulocyte(LDG) population from Systemic Lupus Erythematosis (SLE) patient blood.

“A Distinct Subset of Proinflammatory Neutrophils Isolated from Patients with Systemic Lupus Erythematosus Induces Vascular Damage and Synthesizes Type I IFNs “

M.F. Denny, M.J. Kaplan, et al. (2010) J Immunol 184(6): 3284-3297

This University of Michigan group has used a Biotinylated antibody cocktail in conjunction with anti-Biotin MACS beads to quickly isolate and characterize this unusual population of neutrophils from ficoll prepared, RBC-lysed Systemic Lupus Erythematosus patient blood. Purity of isolated cells was determined as >95% CD10+, CD15+/CD14lo.

Isolated low density granulocytes (LDG) have capacity to interfere with differentiation of Endothelial precursors. While LDG have impaired phagocytic function, they are cytotoxic to vascular endothelium. LDG also exhibit enhanced extracellular trap (NET) ability. This cell population may play a significant role in SLE progression.

Ancell Biotinylated mAbs for negative selection of LDG

anti-CD3, anti-CD7, anti-CD19, anti-CD79b, anti-CD56, anti-MHC Class II, anti-CD86, anti-CD235a

CD64 FcγRI, CD32FcγRII, CD16FcγRIII F(abâ��)2

Ancell Corporation — Mon, 08 Apr 2013 00:00:00 -0500

Featured Products: F(ab’)₂ Fragments of anti-CD64(FcgRI), anti-CD32(FcgRII), and anti-CD16(FcgRIII) for Human Fc Receptor and Immune Complex Research

Low Endotoxin and Essentially Free of Intact Antibody, these dimericantibody fragments block Immunoglobulin binding to their repective receptors

anti-human CD64 (FcgRI)F(ab’)₂ clone 10.1

anti-human CD32 (FcgRII) F(ab’)₂ clone 7.3

anti-human CD16 (FcgRIII) F(ab’)₂ clone 3G8

Literature Citations

Inhibition of Fibrocyte differentiation

D Pilling, N M Tucker, R H Gomer (2006) J Leuk Biol 79(6): 1242-1251.

Modulation of Dendritic cell response to microbial infection

L Romani, P Puccetti, et al. (2004) Int Immunol 16(1): 149-161.

Modulation of Dendritic cell maturation

E A Laborde, M Vulcano, et al. (2007) J Immunol 179(1): 673-81.

anti-CD16 F(ab’)₂Mac-1 upregulation in Neutrophils

Z Xiao, S Neelamegham, et al. (2008) Blood 112: 1091-1100.

Recombinant CD273-muIg

Ancell Corporation — Fri, 29 Mar 2013 00:00:00 -0500

New human biologically active CD273 ( B7-DC, PD-L2) recombinant protein and other related products now available!

Cell Surface Metastatic Markers for Colon Cancer SW480/SW620 Model

Ancell Corporation — Fri, 01 Feb 2013 00:00:00 -0600

SW480 is a primary Colon Epithelial Adenocarcinoma tumor line and SW620 is a metastasized variant of this derived from lymph node. Differential analysis of surface markers may give insight into tumor metastatic potential and/or mechanisms.

“Multiplex Flow Cytometry Barcoding and Antibody Arrays Identify Surface Antigen Profiles of Primary and Metastatic Colon Cancer Cell Lines”

K Sukhedeo, J.N. Rich, et al. (2013) PLOSone 8(1): e53015.

Relevant Ancell Antibody Products

Antigens Upregulated after metastasis

Antigens Downregulated after metastasis

New anti-CD269 (BCMA) antibody availible!

Ancell Corporation — Wed, 05 Dec 2012 00:00:00 -0600

New anti-CD269 (BCMA) antibody availible!

Purified (with antibiotic) catalog # 269-020

Purified (preservative-free) catalog # 269-020

Ancell at AIC in Chicago

Ancell Corporation — Tue, 13 Nov 2012 00:00:00 -0600

Ancell is proud to be a sponsor of the 2012 Autumn Immunology Conference in Chicago Nov 16th -Nov 19th.

Visit us at booth #18!

Clinical Response to Prostate Cancer Immunotherapy using GM-CSF and anti-CD152 Correlates with Diversity of Upmodulated Antibody Binding to Microarray Antigens

Ancell Corporation — Fri, 05 Oct 2012 00:00:00 -0500

“Diversity of Antigen-Specific Responses Induced In Vivo with CTLA-4 Blockade in Prostate Cancer Patients” Kwek S S, L Fong, et al. (Oct 2012) J Immunol 189(7): 3759-3766.

“…advanced prostate cancer patients who clinically respond to treatment also develop enhanced Ab responses to a higher number of Ags than nonresponders…”

Relevant Ancell Products

Anti-CD152(CTLA-4) blocking mAb

Recombinant CD152-muIg

Recombinant CD80-muIg

Recombinant CD86-muIg

CD49d is a useful marker for sorting FoxP3+ human Treg

Ancell Corporation — Mon, 17 Sep 2012 00:00:00 -0500

”CD49d provides access to “untouched” human Foxp3 Treg free of contaminating effector cells” Kleinewietfeld M, K Falk, et al. (2012) Blood 113(4): 827-836.

Magnetic depletion of PBMC using anti-CD127 (Clone hIL7R-M21) and anti-CD49d (Clone BU49) mAbs to yield a pure population of Foxp3 + Treg cells!

Relevant Ancell products:

anti-CD49d (clone BU49)

anti-CD127 (clone ANC8F2)

anti-Interferon gamma

anti-CD4

anti-CD25

PD-1 engagement enhances anti-tumor vaccine in mice

Ancell Corporation — Wed, 05 Sep 2012 00:00:00 -0500

“B7-DC-Ig Enhances Vaccine Effect by a Novel Mechanism Dependent on PD-1 Expression level on T Cell Subsets”

M. Mkrtichyan, S.N. Khleif, et al. (2012) J Immunol 189(5): 2338-2347.

Engagement of CD279(PD-1) by recombinant CD273 (B7-DC)-muIg enhances effect of anti-tumor vaccine in mice.

Two proposed mechanisms by which this recombinant protein may augment anti tumor vaccine effect: 1) It inhibits proliferation of PD-1high CD4+ T cells, thereby reducing the number of T reg cells within the tumor. 2) It decreases the number of exhausted CD8+ T cells which leads to a better tumor specific cytotoxic response.

Related Ancell Products

Anti-human CD279(PD-1) antibody

Recombinant CD274(B7-H1)-muIg

In vivo Treg proliferation from IL-2 signaling alone

Ancell Corporation — Thu, 19 Jul 2012 00:00:00 -0500

Cutting Edge: IL-2 Signals Determine the Degree of TCR Signaling Necessary To Support Regulatory T Cell Proliferation In Vivo.

Zou T, Kambayashi T. et al. J Immunol. 2012 Jul 1;189(1):28-32.

It has been thought that proliferation of T regulatory cells in vivo was dependent on signaling from T cell receptor –MHC Class II interaction. Zou and colleagues report proliferative response of adoptively transferred Treg in MHC knockout recipient mice receiving IL-2 immune complex. A similar effect was observed when STAT5 induced Treg were transferred.

CD4+ CD26(bright) Human T cells display Th17 phenotype

Ancell Corporation — Fri, 25 May 2012 00:00:00 -0500

“Human Th17 Cells Express High Levels of Enzymatically active Dipeptidylpeptidase IV (CD26)”

Bengsch B, R Thimme, et al, (2012) J Immunol 188(11): 5438-5447.

In transwell assays, CD26(bright) Th17 cells inhibited chemotaxis of CXCR4+ Th1 T cells towards CXCL12.

CD26 appears to be an interesting and useful marker for Th17 autoimmunity.

Ancell anti-CD26 reagents (clone 202.36):

Purified anti-CD26

Purified Preservative-free anti-CD26

anti-CD26/Biotin

anti-CD26/FITC

anti-CD26/Phycoerythrin

Citrullinated Protein and RA

Ancell Corporation — Thu, 12 Apr 2012 00:00:00 -0500

A link found between autoimmune antibody specificity to Citrullinated Protein and development of Rheumatoid Arthritis.

“Production of Autoantibodies against Citrullinated Antigens/Peptides by Human B Cells” Maria F. Bellatin, Peter Stastny, et al. (2012) J Immunol 188(7): 3542-3550

Ancell has a large selection of Biotinylated anti-human monoclonal antibodies which would be useful for sorting and/or identifying human B cell populations:

Leptin affects Treg populations in SLE

Ancell Corporation — Mon, 19 Mar 2012 00:00:00 -0500

Levels of the proinflammatory leptin correlated inversely with expansion of CD25+CD4+Foxp3+ T regulatory cells in a Systemic Lupus Erythematosus mouse model (NZB/W). This could lead to a leptin-based immunotherapy.”Cutting Edge: Fasting Induced Hypoleptinemia Expands Functional Regulatory T cells in Systemic Lupus Erythematosus”Yaoyang Liu, Yiyun Yu, Antonio La Cava, (2012) J Immunol 188: 2070-2073.

Relevant Ancell Products:

anti-Human CD25 products

anti-Human CD4 products

anti-Mouse CD4 antibody reagents

SLAM receptors infulence TH17 Differentialtion in SLE

Ancell Corporation — Fri, 03 Feb 2012 00:00:00 -0600

Increased Expression of CD229(SLAMF3) and CD352(SLAMF6)

in SLE T Lymphocytes Promotes Th17 Differentiation

Chatterjee M., G.C. Tsokos, et al. (2012) J Immunol 188: 1206-1212.

Killer Islet Cells!

Ancell Corporation — Tue, 06 Dec 2011 00:00:00 -0600

Transplanted islet cells were biochemically modified to protect their own long term engraftment in a mouse pancreas. These cells have the super-ability to cause apoptosis to occur in the infiltrating pathogenic T cells. This is a novel approach to diabetes treatment!

”Pancreatic Islets Engineered with SA-FasL Protein Establish Robust Localized Tolerance by Inducing Regulatory T Cells in Mice” E.S. Yolcu, H. Shirwan, et al. (2011) J Immunol 187: 5901-5909

CD30-CD153 and CD134(OX40)-CD252 co stimulatory pathways are essential for CD4 dependant autoimmunity

Ancell Corporation — Tue, 01 Nov 2011 00:00:00 -0500

“Abrogation of CD30 and OX40 signals prevents autoimmune disease in FoxP3-deficient mice” FM Gaspal, PJL Lane, et al. (2011) JEM 208(8): 1579-1584.

Ancell related products

Blocking anti-human CD30 mAb

Recombinant human CD134 and CD252 Proteins

Anti-human CD134 and CD252 mAbs

Travel Awards for GLIIFCA 2011

Ancell Corporation — Thu, 29 Sep 2011 00:00:00 -0500

Ancell and Heliocyte are proud to sponsor travel and poster award stipends for Great Lakes International Imaging and Flow Cytometry Association 2011 conference in Oconomowoc, WI!

The need to better understand Treg plasticity and function in the context of autoimmunity

Ancell Corporation — Mon, 12 Sep 2011 00:00:00 -0500

S. Alice Long and Jane H. Buckner “CD4+ FOXP3+ T Regulatory cells in Human Autoimmunity: More than a Numbers Game”

J Immunol (2011) 187:2061-2066

CD30 and CD134 receptors are necessary for CD4 dependent autoimmune tissue damage

Ancell Corporation — Thu, 25 Aug 2011 00:00:00 -0500

Abrogation of CD30 and OX40 signals prevents autoimmune disease in FoxP3-deficient mice. Gaspal F.M., P.J.L Lane, et al. (2011) JEM 208(8): 1579-1584.

Ancell Products

anti-human CD30 antibody products

anti-human CD134(OX40) antibody products

anti-human CD252(OX40L) antibody products

Recombinant human CD134(OX40)

Recombinant human CD252(OX40L)

New anti-Mouse CD4 PE-Cy7 conjugate

Ancell Corporation — Fri, 05 Aug 2011 00:00:00 -0500

New anti-Mouse CD4 PE-Cy7 conjugate available now!

Watch for more PE-Cy7 conjugates available soon.

Ancell announces partnership with HelioCyte!

Ancell Corporation — Mon, 23 May 2011 00:00:00 -0500

Ancell announces partnership with HelioCyte to provide cutting edge Flow Cytometry products!

Watch for new products designed to simplify and improve your Flow research!

Twitter account: Heliocyte

anti-Mouse CD4/DyLight350 conjugate

Ancell Corporation — Wed, 18 May 2011 00:00:00 -0500

Improved multi-color staining of Mouse tissues

Anti-Mouse CD4 (clone GK1.5)/DyLight350* conjugate now available!

Catalog #704-350

DyLight350* is excitable by Near-UV laser Amax 353, and emits in the blue range, Emax 432nm.

Look for more DyLight350 conjugates available soon!

CD134(OX40) Signaling Mechanism Elucidated

Ancell Corporation — Wed, 20 Apr 2011 00:00:00 -0500

Engagement of CD134(OX40) by its Ligand (CD252) induces an intracellular signaling complex which includes PKB, and PI3K, and requires TRAF2.

Takanori S, H Choi, M Croft. (2011) J Immunol 186(6):3547-3555.

Ancell Products:

Recombinant human CD134-muIg Fusion protein

Recombinant human CD252(OX40)-muCD8

Monoclonal anti-human CD134(OX40)

Monoclonal anti-human CD252(OX40L)

CD28 Superagonist protects against Rheumatoid Arthritis mediated bone destruction

Ancell Corporation — Wed, 30 Mar 2011 00:00:00 -0500

In mice, use of CD28 superagonist has been shown to increase the % of Treg cells, and protect from Rheumatoid Arthritis mediated bone destruction.
Marlo M Zaiss, Jean-Pierre David, et al. (2010) J Immunol 184: 7238-7246.

Ancell anti-CD28 antibody clone ANC28.1/5D10 has been characterized as a superagonist.

This mAb has been found to be capable of stimulating IL-2 production from Jurkat cells without T Cell Receptor engagement.

Cutting Edge: Monovalency of CD28 Maintains the Antigen Dependence of T Cell Costimulatory Responses
Kevin M. Dennehy, Thomas Hünig, et al. The Journal of Immunology, 2006, 176: 5725-5729.