Neutrophil Extracellular Trap (NET) is a mechanism by which bacteria and other pathogenic organisms are rendered trapped in a site of inflammation. An activated Neutrophil extrudes it own genomic DNA, rich with histones and other proteins, to trap invading pathogenic organisms. However, in SLE patients, a distinct subset of Neutrophils, Low Density Granulocytes (LDGs) are prevalent. NETosis by LDG are a major component of this autoimmune disorder, due to mitochondria and oxidized products in this extrusion.
“Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.” C Lood, M J Kaplan, et al. (2015) Nature Medicine 22(2): 146-153. PMID: 26779811
Ancell Low Density Neutrophil Research Products
Human LDG Negative Sorting Mix (Set of 8 Biotinylated mAbs which bind all cells except LDGs in an RBC-lysed Ficoll prep)