CD268 (BAFF-R, TNFRSF13C) is a significant marker for B Cell Precursor Acute Lymphoblastic Leukemia (BCP-ALL). Ancell clone ANC268.2 was used to phenotype patient samples. Blocking the BAFF – BAFFR pathway may prove to be useful in treating BCP-ALL.
“TNFRSF13C (BAFFR) positive blasts persist after early treatment and at relapse in childhood B-cell precursor acute lymphoblastic leukaemia.”G Fazio, G Cazzaniga, et al. (2017) BJH doi: 10.1111/bjh. 14794 PMID: 28573703
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The TIGIT – CD155 regulatory pathway is emerging as an important element of immune function. Studies using knockout mice demonstrate that this pathway is important in optimal NK effector cell function (activation and/or tolerance) (1) There is significant potential for the development of immunological drugs that could blockade this pathway, restoring anti-cancer immune response (2).
Relevant Ancell Products
Recombinant TIGIT-muIg (Available Soon!)
Please visit Ancell at booth #812 May 13 -15 at the AAI meetings in Washington DC!
“Endothelial Cell Inflammatory Reactions Are Altered in the Presence of E-Cigarette Extracts of Variable Nicotine” Barber, K.E., Ghebrehiwet, B., Yin, W. et al. (2017) Cel. Mol. Bioeng. 10: 124. doi:10.1007/s12195-016-0465-4
The Authors utilized an in vitro model in which HUVEC (human umbilical cord endothelial cells) were exposed to tobacco vapor/ in HEPES buffer to assess the role of tobacco constituents in causing cardiovascular disease. In the presence of Platelet-poor-plasma, C1q Complement protein deposition was significantly increased in the presence of tobacco vapor, regardless of nicotine content.
Ancell antibodies were used in EIA to assess levels of CD35, CD55 and CD59 molecules, which are known regulators of Complement deposition. CD35 expression was significantly up regulated in the presence of tobacco vapor regardless of nicotine content.
An Ohio research group tested the efficacy of inactivated Swine Influenza virus (SwlV) encapsulated in 200–300 nm diameter PLGA (poly lactic co-glycolic acid) microspheres. Pigs were immunized twice intranasaly. While control pigs had fever for four days, treated pigs showed no clinical flu symptoms.
“Biodegradable nanoparticle delivery of inactivated swine influenza virus vaccine provides heterologous cell-mediated immune response in pigs.” Dhakal, Santosh, G J Renukaradhva, et al. Journal of Controlled Release (2017) 247 (10): 194-205. PMID: 28057521
Ancell Recombinant CD152(CTLA4)-muIg was used to stain porcine APC-expressed CD80 (B7-1) and CD86 (B7-2) in Flow Cytometry.
Recombinant CD152-muIg cat# 501-020
CD36 is a scavenger receptor which can bind to various ligands as well as long chain fatty acids. It plays a role in innate immunity as well as metabolism. The authors use transport inhibitors, and uptake of 14-C labeled Phosphate diester of alpha-Tocopheryl (Vitamin E ) to implicate the CD36/PI3Ky as an important pathway for monocyte activation and subsequent secretion of Vascular Endothelial Growth Factor(VEGF).
“alpha-Tocopheryl Phosphate Induces VEGF Expression via CD36/PI3Ky in THP-1 Monocytes” J M Zingg, A Azzi, M Meydani, J Cell Biochem DOI: 10.1002/jcb.25871 PMID: 28059487
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Visit Ancell at the 2016 Autumn Immunology Conference Nov 18 - 20 at the downtown Marriott in Chicago!
Ancell anti-human FOXP3 mAb and Biotin conjugate now available! Other conjugates coming soon!
Xuehui He, Ruben L Smeets, Esther van Rijssen, Annemieke MH Boots, Irma Joosten, Hans JPM Koenen. (2016) Dissertation Submitted for Publication, Radbound Univ Med Center and Groningen Univ Med Center, The Netherlands.
Ancell anti-CD28 Superagonist Clone ANC28.1/5D10
Adding Low density lipoprotein to FITC labeled Group A Steptococcus increased U-937 phagocytosis rate. This effect was blocked when cells were pre incubated with Ancell anti-CD36 mAb clone SMO.
“LDL acts as an opsonin enhancing the phagocytosis of group A Streptococcus by monocyte and whole human blood” Lulei Zhou, Runlin Han, et al. (2016) Medical Microbiology and Immunology 205(2): 155-162. PMID: 26392394
Relevant Ancell antibodies
Come and visit Ancell at the American Association of Immunologist’s meeting in Seattle May 13 - May 16th, booth #816.
Ask for an Ancell T shirt. They go fast!
anti-CD32 F(ab‘)2 blocked receptors on SLE patient-derived stimulated monocytes and reduced proinflammatory cytokine secretion in vitro
Macrophages derived in culture from SLE patients are directed into secreting proinflammatory cytokines by anti-C1q auto antibodies bound to immobilized C1q complement protein. Pre blocking these monocytes with anti-CD32 (FcgRII ) F(ab’)2 reduced cytokine secretion levels significantly.
“Anti-C1q Autoantibodies from Systemic Lupus Erythematosus Patients Induce a Proinflammatory Phenotype in Macrophages.” Sophia Thanei, Marten Trendelenburg J Immunology (2016) 196: 2063–2074. PMID: 26829984
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Using TALEN(Transcription Activator-Like Effector Nucleases) technology, these researchers from Seattle Children’s Research succeeded in restoring copies of CD154( CD40L) gene with 3’ UTR under control of its endogenous promoter in human T cells from a patient with X-Linked Hyper-IgM Syndrome (X-HIGM).
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Oxidized Mitochondrial products in NETosis are a major component by which LDG drive inflammation in SLE
Neutrophil Extracellular Trap (NET) is a mechanism by which bacteria and other pathogenic organisms are rendered trapped in a site of inflammation. An activated Neutrophil extrudes it own genomic DNA, rich with histones and other proteins, to trap invading pathogenic organisms. However, in SLE patients, a distinct subset of Neutrophils, Low Density Granulocytes (LDGs) are prevalent. NETosis by LDG are a major component of this autoimmune disorder, due to mitochondria and oxidized products in this extrusion.
“Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.” C Lood, M J Kaplan, et al. (2015) Nature Medicine 22(2): 146-153. PMID: 26779811
Ancell Low Density Neutrophil Research Products
Human LDG Negative Sorting Mix (Set of 8 Biotinylated mAbs which bind all cells except LDGs in an RBC-lysed Ficoll prep)
“Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1” S Jutz, P Steinberger, et al. (2016) J Immunol Methods pii: S0022-1759(16)30007-2. PMID: 26780292
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This is distinct from recruitment of Th1 cells where CD162 is also necessary for rolling. CD43 mediated adhesion could provide a means to selectively manipulate Th17 immune response.
“CD43 Functions as an E-Selectin Ligand for Th17 Cells in Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo” Francisco Velazquez, Pilar Alcaide, et al. (Feb 2016) Journal of Immunology 196(3): 1305-1316. PMID: 26700769
Relevant Ancell anti-Human mAb products
These effects are Treg and IL-4 independent, and are instead dependant on the helminth specific Th2-mediated Eosinophilia in which IL-5 and IL-33 cytokines are crucial.
A nice review by the master.
“B10 cells: a functionally defined regulatory B cell subset” T F Tedder (2015) J Immunol 194(4): 1395-1401. PMID: 25663677
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Despite the pronounced proliferative effect of recombinant pentameric GITRL on T cells, MC38 adenocarcinoma tumor failed to regress due to suppressive effect of accumulating Treg in tumor tissue and draining lymph node.
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This Danish research group used a cocktail of Ancell Biotinylated anti tetraspan antibodies, anti-CD9, anti-CD63 and anti-CD81 to detect microvesicles captured on EV microarray. The ability to characterize plasma derived exosomes in a high throughput manner may be very helpful in diagnosing cancer prognosis.
“Potentials and capabilities of the Extracellular Vesicle (EV) Array” Malene Mǿller Jǿrgensen, Rikke Bæk and Kim Varming (2015) Journal of Extracellular Vesicles 4: 26048. PMID 25862471
Ancell Tetraspan mAbs