284-030





*PERFORMANCE DATA SHEET* 0839 Monoclonal Mouse IgG2b/Biotin* Clone:BPC 4 CATALOG#: 284-030 QUANTITY: 100 mg CONCENTRATION: 1.0 mg/ml INFORMATION: Immunoglobulin expressed by the mouse plasmacytoma hybridoma BPC 4 is an IgG2b isotype of unknown specificity. It is used as a negative control when working with human cells. STORAGE CONDITIONS: Store at 2 - 5^oC. Freeze/thawing not recommended. PRODUCT STABILITY: Product should retain activity for at least 12 months after shipping date when stored as recommended. Ship Date:_____________ BUFFER: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN₃ (as a preservative). PRODUCTION: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column. PERFORMANCE: Mouse IgG2b/Biotin was tested as a negative control in Flow cytometry at 5 mg/ml using Streptavidin R-phycoerythrin (Catalog #253-050) as a 2^o reagent. All cells tested failed to stain or display a significant mean shift above a buffer background. Cells tested: Human PBL, Daudi, Jurkat, HPB-MLT, Nalm-6, U937, Molt-4, HUT 78 and Raji (cells expressing Fc receptors were pre-blocked with human IgG). (5-292) *This Product is intended for Laboratory Research use only.