PERFORMANCE DATA SHEET
0839
Monoclonal Mouse IgG2a*
Clone: RPC 5 CATALOG#: 281-010 QUANTITY: 100 CONCENTRATION: 1.0 mg/ml
INFORMATION: Immunoglobulin expressed by the mouse plasmacytoma hybridoma RPC 5 is an IgG2a isotype of unknown specificity. It is used as a negative control when working with human cells.
STORAGE CONDITIONS: Store at 2 - 5oC . Freeze/Thawing is not recommended.
PRODUCT STABILITY: Product should retain activity for at least 12 months after shipping date when stored as recommended. Ship Date:_____________
BUFFER: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
PRODUCTION: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.
PERFORMANCE: Mouse IgG2a was tested as a negative control in Flow cytometry at 20 mg/ml using Goat anti-Mouse IgG/FITC (Cat # 232-010) as a 2o reagent. All cells tested failed to stain or display a significant mean shift above a buffer background.
Cells tested: Human PBL, HPB-MLT, Nalm-6, Raji, HL60 and KG-1 (cells were pre-blocked with human IgG to prevent nonspecific binding). (11-181)
*This Product is intended for Laboratory Research use only.