PERFORMANCE DATA SHEET

0839

Monoclonal Mouse IgG1/FITC*

Clone:MOPC 31C

CATALOG#: 278-040

CONCENTRATION: 0.5 mg/ml

QUANTITY: 100 mg

INFORMATION: Immunoglobulin expressed by the mouse plasmacytoma MOPC 31C is an IgG1k isotype of unknown specificity. It is used as a negative control when working with human cells.

STORAGE CONDITIONS: Store at 2 - 5^o C. Freeze/Thawing is not recommended. Protect from light.

PRODUCT STABILITY: Product should retain activity for at least 12 months after shipping date when stored as recommended. Ship Date:_____________

BUFFER: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN₃ (as a preservative).

PRODUCTION: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/Antibody molar ratio of approximately 9.0.

PERFORMANCE: MIgG1/FITC was tested as a negative control in Flow cytometry at 20 mg/ml. All cells tested failed to stain or display a significant mean shift above a buffer background.

Cells tested: Human PBL, CEM, HPB-MLT, U-937, Nalm-6, and Raji (cells expressing Fc receptors were pre-blocked with human IgG). (10-64)

 *This Product is intended for Laboratory Research use only.