PERFORMANCE DATA SHEET

0839

Monoclonal anti-mouse CD8 alpha(Lyt 2)*

mAb name/Clone:53-6.72

Isotype:Rat IgG2ak

Immunogen: murine thymocytes/splenocytes

CATALOG#: 260-020

QUANTITY: 100 mg

CONCENTRATION: 1.0 mg/ml

INFORMATION: Antibody 53-6.72 binds to the alpha subunit of murine CD8 for all mouse strains tested. It is a useful tool for assessing murine CD8a containing recombinant proteins.

References: 1) Ledbetter, J.A., et al. (1979) Immunol. Rev. 47: 63-90.

STORAGE CONDITIONS: Store at 2 - 5^o C. Freeze/Thawing is not recommended.

PRODUCT STABILITY: Product should retain activity for at least 12 months after shipping date when stored as recommended. Ship Date:_____________

BUFFER: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).

PRODUCTION: Antibody was Protein G purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.

PERFORMANCE: Reagent was tested as a 2 ^o staining reagent for use with CD8 alpha containing recombinant fusion proteins. Optimal signal was observed when 5 x 10⁵ cells per tube were incubated 45 minutes on ice with 80m l of anti-mouse CD8 alpha antibody at a concentration of 10 mg/ml, after which they were washed twice and incubated with Goat anti-Rat IgG/FITC. Cells were washed three times, fixed and analyzed using a BD FACS  Calibur.

*This Product is intended for Laboratory Research use only.