PERFORMANCE DATA SHEET

0839

Monoclonal anti-mouse CD8 alpha(Lyt 2)/R-PE*

mAb name/Clone:53-6.72

Isotype:Rat IgG2a k

Immunogen: murine thymocytes/splenocytes

CATALOG#: 260-050

QUANTITY: 120 tests

VOLUME IN VIAL: 0.2 ml

WORKING DILUTION: 1:50 (80ml/test)

INFORMATION: Antibody 53-6.72 binds to the alpha subunit of murine CD8 for all mouse strains tested. It is a useful tool for assessing murine CD8a containing recombinant proteins.

References: 1) Ledbetter, J.A., et al. (1979) Immunol. Rev. 47: 63-90.

STORAGE CONDITIONS: Store at 2 - 5^o C. Do not freeze! Protect from light.

PRODUCT STABILITY: Product should retain activity for at least 12 months after shipping date when stored as recommended. Ship Date:_____________

BUFFER: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN₃ (as a preservative).

PRODUCTION: Protein G purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 ratio of 2.82.

PERFORMANCE: Reagent was tested as a 2^o staining reagent for use with CD8 alpha containing recombinant fusion proteins. Optimal signal was observed when 5 x 10⁵ cells per tube were incubated 45 minutes on ice with 80ml of anti-mouse CD8 alpha/R-PE at a dilution factor of 1:50. Cells were washed three times, fixed and analyzed using a BD FACS Calibur. (9-279)

*This Product is intended for Laboratory Research use only. R-Phycoerythrin (R-PE) is covered under patents: U.S. 4,520,110; European 76,695 and Canadian 1,179,942.