Human CD95L-muCD8 Fusion Protein

Human FAS ligand (CD95L) is a 40kd type II membrane protein that induces apoptosis in cells expressing FAS (CD95) (1, 2).  Activated human T cells release bioactive FAS ligand microvesicles (3).  FAS ligand is one of the major effectors of CD8⁺CTL and NK activity.  Loss of FAS ligand leads to lymphoproliferative and autoimmune disease.

Molecular Structure: A soluble molecule consisting of the extracellular domain (177aa) of human CD95L fused  to the extracellular domain (167aa) of murine CD8 alpha (2), with a predicted monomeric molecular weight of 38.9 kd.

Transfectant Cell Line:  CHO

Immunochemistry Applications:   Flow cytometry       Blocking        Elisa        Frozen Sections  

Functional Application:   1 ug/ml of  CD178-muCD8 will induce apoptosis in 1 x 10⁵ human Jurkat cells in 100 ul culture volume in an overnight assay. 

Human CD178-muCD8 binding to CD95 positive cells is detectable in Flow cytometry when co stained with anti-mouse CD8a/PE (cat# 260-050).

References:

1.   T. Takahashi, et al, (1994) Intl Immunol  6: 1567-1574. 

2.  T. Suda, et al, (1997) J Exp Med  186: 2045-2050. 

3.   M.J. Martinez-Lorenzo, et al, (1999) J Immunol 163: 1274-1281.

4. H Wajant, (2003) Essays Biochem 39:53-71.

Catalog Number:

511-020-Purified (With Antibiotics) - 25ug 

511-820 -Purified (Preservative Free) - 25ug 

511-030-Biotinylated - 25ug